The resulting model is consistent with results from site-directed mutagenesis experiments and shows the beta-D-glucose molecule in the active site of glucose oxidase, stabilized by 12 hydrogen bonds and by hydrophobic contacts to three neighbouring aromatic residues and to flavin adenine dinucleotide. niger were modelled by force-field methods. Substrate complexes of the enzyme from A. deviation of 0.7 A for main-chain atoms and show four N-glycosylation sites, with an extended carbohydrate moiety at Asn89. The structures of the partially deglycosylated enzymes have an r.m.s. The structure of the enzyme from Aspergillus niger, previously refined at 2.3 A resolution, has been refined at 1.9 A resolution to an R value of 19.0%, and the structure of the enzyme from Penicillium amagasakiense, which has 65% sequence identity, has been determined by molecular replacement and refined at 1.8 A resolution to an R value of 16.4%. Glucose oxidase is a flavin-dependent enzyme which catalyses the oxidation of beta-D-glucose by molecular oxygen to delta-gluconolactone and hydrogen peroxide. Diversity, Equity, Inclusion, and Access.Citation, Usage, Privacy Policies, Logo.Biologically Interesting Molecule Reference Dictionary (BIRD).In the articles in the series, to be published in this journal, outstanding experts in their respective fields will give (a) an overview on topics related to the practical use of biotechnological methods in organic chemistry, (b) an outlook on upcoming research topics, their impact on existing areas and their potential for future developments.The Commission solicits comments as well as suggestions for future topics, and will aim to help in providing answers to any questions in this field. By improving the knowledge of chemists in the field of biotechnology it is hoped to initiate more ideas for applying biological methods in chemistry, inspire more use of chemical knowledge in the biological sciences and help scientists in both fields to work closer together.
Biotransformation - a Useful Tool in Organic ChemistryThe Commission has chosen this series with a view to intensify the interrelations of chemistry and biotechnology. Interrelations of Chemistry and Biotechnology - I.
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